Assisi Animal Health is committed to providing clinical solutions, backed by research and evidence-based data. Our double-blind, peer-reviewed veterinary clinical studies are at the heart of all that we do. These studies firmly root Assisi in the scientific veterinary community by verifying that our proprietary targeted pulsed electromagnetic field (tPEMF™) technology significantly reduces pain and inflammation in animals. We’ve taken the research one step further, with the development of a newtPEMF signal created to reduce anxiety in dogs. We explored the potential for this new signal in our 2019 pilot study, which examined the efficacy of Assisi’s new Calmer Canine device in treating dogs with separation anxiety. This groundbreaking study demonstrates that the unique tPEMF signal used in the Calmer Canine device reduces canine anxiety and restores calm.

Peer-Reviewed Veterinary Clinical Studies: Proving the Effect of the Assisi Loop®

Study published in the Journal of the American Animal Hospital Association, 20191

This double-blind, randomized, placebo-controlled clinical trial found that using the Assisi Loop to treat dogs suffering from intervertebral disk disease (IVDD) resulted in:

  • Significant improvement in wound healing for Loop treated group compared to placebo
  • Reduction in opioid administration: opioids were administered almost 50% less frequently in Loop treated group
  • No side effects

Study published in the Journal of Neurotrauma, 20182

This randomized, placebo-controlled, prospective clinical trialfound that using the Assisi Loop to treat dogs with spinal cord injuries (SCI) due to acute intervertebral disc extrusions (IVDE) resulted in:

  • Significant difference in incisional pain between Loop treated and placebo group
  • Lower levels of GFAP indicatingneuroprotective qualities
  • Statistically greater degree of recovery of proprioception

Study published in the Journal of the American Animal Hospital Association, 20191

This double-blind, randomized, placebo-controlled clinical trial found that using the Assisi Loop to treat dogs suffering from intervertebral disk disease (IVDD) resulted in:

Significant improvement in wound healing for Loop treated group compared to placebo

Reduction in opioid administration: opioids were administered almost 50% less frequently in Loop treated group

No side effects

Study published in the Journal of Neurotrauma, 20182

This randomized, placebo-controlled, prospective clinical trialfound that using the Assisi Loop to treat dogs with spinal cord injuries (SCI) due to acute intervertebral disc extrusions (IVDE) resulted in:

Significant difference in incisional pain between Loop treated and placebo group

Lower levels of GFAP indicatingneuroprotective qualities

Statistically greater degree of recovery of proprioception

1 Leilani X. Alvarez, John McCue, Nathaniel K. Lam, Gulce Askin, and Philip R. Fox (2019) Effect of Targeted Pulsed Electromagnetic Field Therapy on Canine Postoperative Hemilaminectomy: A Double-Blind, Randomized, Placebo-Controlled Clinical Trial. Journal of the American Animal Hospital Association: March/April 2019, Vol. 55, No.

2Zidan N, Fenn J, Griffith E, Early PJ, Mariani CL, Muñana KR, Guevar J, Olby NJ. J Neurotrauma. 2018 Aug 1;35(15):1726-1736. doi: 10.1089/neu.2017.5485. Epub 2018 Apr 2.

Peer-Reviewed Veterinary Clinical Studies: Proving the Effect of the Assisi Loop®

Study published in the Journal of the American Animal Hospital Association, 20191

This double-blind, randomized, placebo-controlled clinical trial found that using the Assisi Loop to treat dogs suffering from intervertebral disk disease (IVDD) resulted in:

Significant improvement in wound healing for Loop treated group compared to placebo

Reduction in opioid administration: opioids were administered almost 50% less frequently in Loop treated group

No side effects

Study published in the Journal of Neurotrauma, 20182

This randomized, placebo-controlled, prospective clinical trialfound that using the Assisi Loop to treat dogs with spinal cord injuries (SCI) due to acute intervertebral disc extrusions (IVDE) resulted in:

Significant difference in incisional pain between Loop treated and placebo group

Lower levels of GFAP indicatingneuroprotective qualities

Statistically greater degree of recovery of proprioception

1) Leilani X. Alvarez, John McCue, Nathaniel K. Lam, Gulce Askin, and Philip R. Fox (2019) Effect of Targeted Pulsed Electromagnetic Field Therapy on Canine Postoperative Hemilaminectomy: A Double-Blind, Randomized, Placebo-Controlled Clinical Trial. Journal of the American Animal Hospital Association: March/April 2019, Vol. 55, No.

2) pp. 83-91.2Zidan N, Fenn J, Griffith E, Early PJ, Mariani CL, Muñana KR, Guevar J, OlbyNJ. J Neurotrauma. 2018 Aug 1;35(15):1726-1736. doi: 10.1089/neu.2017.5485. Epub 2018 Apr 2.

Separation Anxiety Veterinary Clinical Study: Demonstrating the Effect of the Calmer Canine™

Pilot Study, Presented July 2019 at the IVBM Conference, Washington DC.3

This pilot study testing the efficacy of the Calmer Canine device on dogs with separation anxiety found that:

  • After a month of treatment, 100% of cases met the primary and secondary endpoints, which were improvement in overall severity score based on owner assessment and improvement in clinical signs based on video analysis. Even accounting for a 50% placebo effect, this data is statistically significant.
  • 56%of cases had at least a one-point reduction in their overall severity score, as evaluated by their owners after only 1 week of therapy.
  • After six weeks of therapy, 50% of owners reported their dogs resolved of CSA signs irrespective of their starting point.
  • Video data revealed that nearly 80% of dogs in the study had at least one sign improve more than 50% and half of the dogs saw at least one CSA sign disappear after a month of treatment with Calmer Canine.
  • No side effects were reported.
  • The signs of CSAcontinued to improve or remained at their reduced level in two-thirds of the dogs tested, even after treatment had been discontinued for two weeks.

Watch before and after video footage from the pilot study.

The Research Continues...

Assisi, in collaboration with North Carolina State University, is conducting a larger, double-blind, placebo-controlled study to further support the significant results found in the pilot study. This new study aims to enroll 40 dogs with moderate to severe separation anxiety with similar efficacy endpoints to the pilot study. Dr. Margaret Gruen and Dr. Katherine Pankratz are the lead investigators.

Human Clinical Studies: The History of tPEMF

In 2010 the FDA cleared PEMF technology for use on humans. In the past decade, research into its efficacy has been mounting in both human and animal science. Since 2013, Assisi’s clinical studies have been based on tPEMF technology and its effectiveness in pain management, advancing healing, and control of anxiety. Some of the most significant human studies are listed below, along with their conclusions with regard to the benefits of tPEMF therapy:

  • Accelerated wound closure(Kloth, et al., Electricity and Magnetism in Biology and Medicine, 1999)4
  • Angiogenic (blood vessel forming) effect (Weber, et al., Plastic and Reconstructive Surgery, 2004)5
  • Increased tensile (maximum) strength (Strauch, et al., J Hand Surg, 2006)6
  • Reduced pain and edema(Pilla, A., Bioelectromagnetic Society Meeting, 2008)7
  • Accelerated surgical recovery (Rohde, C., et al., Plastic and Reconstructive Surgery, 2010)8
  • Reduced arthritic pain (Nelson, Pilla, et al., Rheumatology Int, 2012)9
  • Substantial improvement observed in mood following low field magnetic stimulation treatment (Rohan, et al., Biological Psychiatry, 2013)10
  • Accelerated surgical recovery and reduced inflammation (Rhode, C., et al., Plastic and Reconstructive Surgery, 2015)11

Citations for Human Research with Links